Eastern Conference[]
- Chicago Fire
- Columbus Crew
- D.C. United
- Kansas City Wizards
- New England Revolution
- New York Red Bulls
- Toronto F.C.
Western Conference[]
- C.D. Chivas USA
- Colorado Rapids
- F.C. Dallas
- Houston Dynamo
- Los Angeles Galaxy
- Real Salt Lake
- San Jose Earthquakes
Expansion Teams[]
- Seattle Sounders FC (2009)
- Philadelphia MLS (2010)
Former Teams[]
- Miami Fusion
- Tampa Bay Mutiny